CORDELIER Sylvain

Contact details

Associate Professor at the University of Reims Champagne Ardenne

Faculty of Exact and Natural Sciences, Research Unit on Grapevine and Wines in Champagne, URVVC, EA 4707

Moulin de la Housse - Bâtiment 18 - BP 1039- 51687 Reims Cedex 2 France

Phone number: 00 33 3 26 91 82 01

Fax: 00 33 3 26 91 87 10

Email: sylvain.cordelier@univ-reims.fr

Research activities

My research goals are related to the biostimulation by bacterial elicitors of the growth stimulation and diseases resistance of plant. One objective is to elucidate the molecular, biochemical and cellular events involved in the cross talk between the elicitor and the host plant leading to the establishment of the induced resistance. This induced resistance is evaluated by measurement of the protection level of grapevine against Botrytis cinerea through defence’s induction after treatment with purified bacterial molecules. This study allowed the identification of protective markers by proteomic approaches and their validation is underway by genetic approaches in grapevine but also in Arabidopsis and tomato. This induced resistance is also studied at local and systemic level (organ dependent) after the perception of the bacterial elicitors.

Research keywords

induced resistance, Botrytis cinerea, plant pathology, genetic modification, phytoalexins

Technical competences

Genetics: genotyping, mutants screening, lines introgression followed by field trials

Molecular biology: Northern blot, Southern blot, production and screening of cosmid and BAC libraries, RAPD, PCR, sequencing, cloning, expression and detection of RNAi

Microbiology: Plant cell cultures (Arabidopsis, tobacco, grapevine), genetic modification of Arabidopsis, tobacco, tomato and corn, bacteriology, manipulation of plant pathogens Protein biochemistry: Protein purification by FPLC, enzymology, Western blot

Metabolite analysis: High Performance Liquid Chromatography (HPLC)

Teaching activities

220 hours of teaching performed, including 130 hours of practical work.

Elected member of the Council of Biology-Biochemistry Department of Exact and Natural Sciences Faculty of the University of Reims Champagne-Ardennes (2013-).

Member of the school board of the mention of Master "Production and Valorisation of Agro-Resources" (2013-)

Coordinator of Master 2 "Improvement and Production of Agro-Resources" (2008-2012)

Responsible of several educational modules at Bachelor and Master level (Plant Biotechnology, Tissue Culture, Crop Protection, Company Training, Professional Project).

Teaching keywords

Plant physiology, plant biotechnology, tissue culture, crop protection, botany

Coordination of research programs

Supervision of a doctoral and post-doctoral students financed by the regional program Vinéal2

Collaborations: Max-Planck-Institute for Plant Breeding Research, Köln, Germany ; INRA Colmar ; INRA

Avignon ; Institut de Biologie de l'Ecole Normale Supérieure de Paris

5 recent publications

- Jeandet, P.; Hébrard, C.; Deville, M.A.; Cordelier, S.; Dorey, S.; Aziz, A.; Crouzet, J. (2014) Deciphering the role of phytoalexins in plant-microorganism interactions and human health. Molecules, 19, 18033-18056 doi:10.3390/molecules191118033. (FI=2,09)

- Delaunois B, Jeandet P, Clément C, Baillieul F, Dorey S, Cordelier S. (2014) Uncovering plant-pathogen crosstalk through apoplastic proteomic studies. Frontiers in Plant Science5:249. doi: 10.3389/fpls.2014.00249.(FI=3,6)

- Le Hénanff G, Profizi C, Courteaux B, Rabenoelina F, Gérard C, Clément C, Baillieul F, Cordelier S*, DhondtCordelier S*. (2013) Grapevine NAC1 transcription factor as a convergent node in developmental processes, abiotic stresses, and necrotrophic/biotrophic pathogen tolerance.Journal of Experimental Botany 64(16):4877- 93. doi: 10.1093/jxb/ert277. *Co-last authors (FI=5,24)

- Delaunois B, Farace G, Jeandet P, Clément C, Baillieul F, Dorey S, Cordelier S.(2013) Elicitors as alternative strategy to pesticides in grapevine? Current knowledge on their mode of action from controlled conditions to vineyard. Environnemental Science and Pollution Research. doi: 10.1007/s11356-013-1841-4. (FI=2,61)

- Delaunois B, Colby T, Belloy N, Conreux A, Harzen A, Baillieul F, Clément C, Schmidt J, Jeandet P, Cordelier S. (2013) Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes, BMC Plant Biology, 13:24 doi:10.1186/1471-2229-13-24. (FI=4,35)

5 recent international conferences

- Jeandet P, Adrian M, Clément C, Cilindre C, Hong YS, Cordelier S, Chastang T, Gougeon R, Boutegrabet L, Schmitt-Kopplin P, Courot E. (2013)Analyzing hydroxystilbenes in grape and wine: resveratrol, a playground for analysts? In Vino Analytica Scientia Symposium, Université de Reims, France

- Cordelier S, Le Hénanff G, ProfiziC, Courteaux B, Rabenoelina F, Gérard C, Clément C, Baillieul F, DhondtCordelier S. (2013) Le facteur de transcription de vigne VvNAC1 : un nœud de convergence dans les processus de développement et la tolérance au froid et à B. cinerea.1er Colloque du Réseau Vigne et Vins Septentrional, Colmar, France

- Delaunois B, Colby T, Belloy N, Conreux A, Harzen A, Baillieul F, Clément C, Schmidt J, Jeandet P, Cordelier S. (2011) Snapshot of the grapevine secretome using a proteomic approach. Congress on PR-Proteins and Induced Resistance against Pathogens and Insects, Université de Neuchâtel, Switzerland.

- Delaunois B, Colby T, Belloy N, Conreux A, Harzen A, Baillieul F, Clément C, Schmidt J, Jeandet P, Cordelier S. (2011) Identification of putative protection markers in Vitis vinifera against Botrytis cinerea. Congress on PRProteins and Induced Resistance against Pathogens and Insects, Université de Neuchâtel, Switzerland.

- Jeandet P, Delaunois B, Cordelier S, Adrian M, Pannetier B, Clément C, Courot E. (2011) Biological activity and anticancer effects of resveratrol and related plant stilbenes. EPS Anticancer Drugs Conference 2011, Stockholm, Sweden